Hirschsprung's Disease

	Ganglion cells (arrow) are demonstrated in normal bowel segment
	Only thick nerve fiber is demonstrated without ganglion cell in Hirschsprung's disease
	Noblett type suction biopsy set
	Suction rectal biopsy is done without any anesthesia
	A tiny piece of rectal tissue obtained from suction biopsy including mucosa and submucosa is sufficient for Acetylcholinesterase activity study
	Normal acetylcholinesterase activity in normal specimen
	Increased acetylcholinesterase activity in Hirschsprung's disease, thick staining is observed in mucosa and submucosa layer
	Increased acetylcholinesterase activity in Hirschsprung's disease, thick staining is observed in mucosa and submucosa layer
	Increased acetylcholinesterase activity in Hirschsprung's disease, thick staining is observed in mucosa and submucosa layer (LP: lamina propria, MM: muscularis mucosae, SM: submucosa)
	Increased acetylcholinesterase activity in Hirschsprung's disease, thick staining is observed in mucosa and submucosa layer (LP: lamina propria, MM: muscularis mucosae, SM: submucosa)