M0   Acute Myelocytic Leukemia, undifferentiated

 

Criteria	ANLL, M0  1. Large, agranular blasts (resemble ALL L2, rarely L1)  2. Myeloperoxidase negative or < 3 percent positive;      B-,T lineage markers negative;  3. CD13 and/or CD33 positive; Myeloperoxidase positive by      immunochemistry or electron microscopy; TdT may be positive
Peripheal Blood Smear	ภาพแสดง myeloblast  ใน  ANLL M0    ภาพแสดง blast ใน ANLL M0 ย้อม immunoperoxidase ติด CD 13
Bone Marrow Study	ภาพแสดง agranular blast ใน  ANLL M0   ภาพแสดง blast ใน ANLL M0 ย้อม immunoperoxidase ติด CD 13

 

M1 Acute Myelocytic Leukemia without maluration

 

Criteria	ANLL M1  1. Blast cells, agranular and granular types (type I and II) >90 percent of non-erythroid cells.  At least 3 percent of these are peroxidase or Sudan black positive  2. Remaining 10 percent (or less) of cells are maturing granulocytes or monocytes
Peripheal Blood Smear
Bone Marrow Study

 

M2  Acute Myelocytic Leukemia  with maluration

 

Criteria	ANLL M2  1. Sum of agranular and granular blasts (types I and II) is from  30 to 89 percent of non-erythroid cells  2. Monocytic cells <20 percent  3. Granulocytes from promyelocytes to mature neutrophil  >10 percent
Peripheral Blood Smear
Bone Marrow Study

 

M3  Acute  Promyelocytic Leukemia (APL)

 

Criteria	ANLL M3  1. Majority of cells are abnormal promyelocytes with heavy granulation  2. Characteristic cells containing bundles of Auer rods (‘faggots’) invariably present.  Note: Microgranular variant also occurs ( ANLL,M3v)
Peripheal Blood Smear	M3        M3 variant (M3v)
Bone Marrow Study	M3
	ภาพ BM ของ ANLL M3 under phase  contrast แสดง granulues ที่มีขนาดใหญ่และมากกว่าปกติ

M4  Acute Myelomonocytic Leukemia (AMMoL)

 

Criteria	ANLL, M4  1. In the marrow, blasts>30 percent of non-erythroid cells.  2. Sum of myeloblasts, promyelocytes,  myelocytes and later granulocytes is between 30 and 80 percent of non-erythroid cells  3. >20 percent of non-erythroid cells are monocyte  lineage  4. If monocytic cells exceed 80 percent, diagnosis  is M5  ANLL M4 with Eosinophilia  1. Eosinophils >5 percent of non-erythroid cells in marrow  2. Eosinophils are abnormal  3. Eosinophils chloroacetate and PAS positive
Peripheal Blood Smear	M4    M4  with Eosinophilia
Bone Marrow Study	M4  M4 with Eosinophilia

 

M5  Acute Monoblastic Leukemia (AMoL)

 

Criteria	ANLL M5  1. 80 percent of marrow non-erythroid cells are monoblasts, promonocytes or monocytes  2. M5a , 80 percent of monocytic cells are  monoblasts  3. M5b , <80 percent of monocytic cells are monoblasts, remainders are predominant promonocytes and monocytes
Peripheal Blood Smear	ภาพ ANLL M5a  แสดง ภาพ Monoblasts 3 ตัว   ภาพ ANLL M5b  แสดงภาพ Promonocytes   ภาพ ANLL M5b  แสดง abnormal monocytes
Bone Marrow Study	M5a   M5b

 

M6  Acute Erythroblastic Leukemia (AEL)

 

Criteria	ANLL M6  1. The erythroid component of the marrow exceeds 40 percent of all nucleated cells  2. 30 percent of the remaining non-erythroid cells are agranular or granular blasts (types I and II)  Note; if >50 percent erythroid cells but <30 percent blasts, diagnosis becomes myelodysplastic syndrome
Peripheal Blood Smear	ภาพ PBS ของ ANLL M6  พบ abnormal proerythroblast  ซึ่งมี vacuolation มาก
Bone Marrow Study

 

M7  Acute Megakaryocytic Leukemia (AMegL)

 

Criteria	ANLL M7  1. 30 percent at least of nucleated cells are blasts  2. Blasts identified by platelet peroxidase on electron  microscopy, or by monoclonal antibodies.  3. Increased reticulin is common
Peripheal Blood Smear
Bone Marrow Study