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A. |
¡ÒÃÂéÍÁ¿ÔÅìÁàÅ×Í´´éÇ Wright's stain ÇÔ¸Õ¹Õé¢Ñ鹵͹¡Òà fix áÅСÒÃÂéÍÁᡡѹ ´Ñ§¹Ñ鹡ÒÃÂéÍÁ¿ÔÅìÁàÅ×Í´ª¹Ô´ºÒ§ µéͧ·Ó¡Òà fix ¡è͹´éÇ absolute methanol |
ã¹ Wright’s stain »ÃСͺ´éÇ fixative áÅÐÊÕ¼ÊÁ¡Ñ¹ÍÂÙè ´Ñ§¹Ñ鹡ÒÃÂéÍÁ¿ÔÅìÁàÅ×Í´´éÇ Wright’s stain ¨Ð·Ó¡Òà fix áÅÐÂéÍÁã¹àÇÅÒà´ÕÂǡѹ |
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B. |
¡ÒÃàµÃÕÂÁ¿ÔÅìÁàÅ×Í´ÊÒÁÒöàµÃÕÂÁä´é¨Ò¡àÅ×Í´Ê´æ ·Ñ駪¹Ô´·Õèà¡çºâ´ÂãÊè anticoagulant (EDTA) áÅÐäÁèãÊè anticoagulant |
anticoagulant (EDTA) à»ç¹ÊÒáѹäÁèãËéàÅ×Í´á¢ç§µÑÇ ã¹¡Ã³Õ·Õèµéͧ¡Ò÷ÓÊäÊ´ì¨Ó¹Ç¹ÁÒ¡¨Ò¡µÑÇÍÂèÒ§àÅ×Í´·Õèà¨ÒШҡ venous blood à¾×èÍ¡ÒÃàÃÕ¹¡ÒÃÊ͹ ¡ç¨Óà»ç¹µéͧãªé äÁèÁռŵèÍ¡ÒõÃǨËÒàª×éÍ»ÃÊÔµ |
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C. |
ÊäÅ´ì·ÕèãªéàµÃÕÂÁ¿ÔÅìÁàÅ×Í´ËÒ¡à»ç¹ÊäÅ´ìãËÁè¹ÓÁÒãªéä´éàÅ áµè¶éÒà»ç¹ÊäÅ´ìà¡èÒµéͧäÁèÁÕÃÍ¢մ¢èǹ ¤ÇÃÅéҧ㹹éÓÂÒÅéÒ§ÊäÅ´ì áÅéÇáªèã¹ 70% ethyl alcohol |
ÊäÅ´ìãËÁè·Õè¨Ð¹ÓÁÒãªéàµÃÕÂÁ¿ÔÅìÁàÅ×Í´¤ÇÃáªèã¹ alcohol ¡è͹¹ÓÁÒãªéàªè¹¡Ñ¹ ·Ñ駹Õéà¾×èÍãËéä´é¼Å´Õ㹡ÒõÃǨÇÔ¹Ô¨©Ñ |
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D. |
¡ÒÃÂéÍÁ¿ÔÅìÁàÅ×Í´´éÇ Wright's stain áÅÐ Giemsa stain ãËé¼Å´Õà·èÒà·ÕÂÁ¡Ñ¹ áµè Wright's stain ãªéàÇÅÒ¹éÍ¡ÇèÒ ¢Ñ鹵͹äÁèÂØè§ÂÒ¡¨Ö§à»ç¹·Õè¹ÔÂÁÁÒ¡¡ÇèÒ |
¡ÒÃÂéÍÁ¿ÔÅìÁàÅ×Í´´éÇ Giemsa stain ãËé¼Å´Õ¡ÇèÒ à¾ÃÒÐÊäÅ´ì·ÕèÂéÍÁáÅéÇà¡çºäÇéä´é¹Ò¹¡ÇèÒÁÒ¡â´ÂÁÕÊÕäÁèà»ÅÕè¹á»Å§àÁ×èÍà·Õº¡Ñº Wright’s stain áÅСÒÃÂéÍÁ¿ÔÅìÁàÅ×ʹ˹ҴéÇÂÇÔ¸ÕËÅѧ¹Õé ÁÑ¡ãËé¼ÅäÁè´Õà·èÒÊäÅ´ì·ÕèÂéÍÁ´éÇ Giemsa stain áÅÐÁÕ¢éͤÇÃÃÐÇѧ·Õè¨ÐäÁèãËéà¡Ô´µÐ¡Í¹ÊÕº¹ÊäÅ´ìã¹¢Ñ鹵͹ÊØ´·éÒ¢ͧ¡ÒÃÅéÒ§ÊÕ |
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E. |
ÊÕ Giemsa working solution àÁ×èÍãªéÂéÍÁáÅéÇÊÒÁÒöà¡çºäÇéãªéä´éÍÕ¡ËÅÒ¤ÃÑé§à»ç¹àÇÅÒ¹Ò¹ |
ÊÕ Giemsa ·Õèà¨×ͨҧ (working solution) áÅÐãªéÂéÍÁáÅéÇ ÊÒÁÒöãªéä´éÍÕ¡ÀÒÂã¹Çѹ·ÕèàµÃÕÂÁ ¤×ÍÁÕÍÒÂØ¡ÒÃãªé§Ò¹à¾Õ§ 1 Çѹ ¨Ö§¨ÐãËé¼Å´Õ |